Abstract: The proper choice of raw materials and manufacturing parameters for Cross Laminated Timber is essential to promote the proper bonding of the lamellas, aiming at their application in construction. However, few Brazilian species are currently used in the production of Cross Laminated Timber. The aim of this work was to characterize the bonding quality of four Brazilian reforestation species (Pinus elliottii (pino ellioti), Eucalyptus grandis (eucalipto), Toona ciliata (toona) and Acrocarpus fraxinifolius (lazcar)) in the manufacture of Cross Laminated Timber with two-component polyurethane adhesive and five different pressure levels (from 0,1 MPa to 1,3 MPa). Bonding quality was evaluated through delamination and glue line shear tests based on the European standard. Delamination was affected by the wood species, and the best adhesion occurred for the Cross Laminated Timber manufactured with Pinus elliottii (pino ellioti) and Acrocarpus fraxinifolius (lazcar) at the bonding pressure of 0,7 MPa. Cross Laminated Timber bonding pressure did not affect the percentage of wood in the shear test fractured surface, whereas higher density Cross Laminated Timber showed higher percentages.

Resumen: Este estudio tuvo como objetivo identificar y comprender las representaciones sociales de la vejez LGBTI entre adultos en una comunidad rural costera. Hubo 31 personas, en su mayoría mujeres (65%), heterosexuales (97%), católicos (97%), pescadores (42%), con una edad promedio de 48 años. Se llevaron a cabo entrevistas semiestructuradas, analizadas con el software IRaMuTeQ, versión 0.7 alpha 2, con el método de Clasificación jerárquica descendente. Se identificaron representaciones dicotómicas sobre los constructos estudiados, que muestran signos de prejuicios sutiles dirigidos a las personas mayores de LGBTI, así como una comprensión biologizante del envejecimiento. Los resultados fueron discutidos, corroborando y / o contrastando con estudios previos. Se espera que este estudio contribuya al desarrollo de investigaciones futuras y a la creación e implementación de políticas públicas dirigidas a esta población.

Abstract: This study aimed to identify and understand the social representations of LGBTI old age among adults in a coastal rural community. There were 31 people, mostly women (65%), heterosexuals (97%), Catholics (97%), fishermen (42%), with an average age of 48 years. Semi-structured interviews were carried out, analyzed using the IRaMuTeQ software, version 0.7 alpha 2, using the Descending Hierarchical Classification method. Dichotomous representations about the studied constructs were identified, which bear signs of subtle prejudice directed at LGBTI’s elderly people, as well as a biologizing understanding of aging. The results were discussed, corroborating and / or contrasting them with previous studies. It is expected that this study will contribute to the development of future research, and to the creation and implementation of public policies aimed at this population.

Resumo: Esse estudo teve como objetivo identificar e compreender as representações sociais da velhice LGBTI entre pessoas adultas de uma comunidade rural litorânea. Contou-se com a participação de 31 pessoas, majoritariamente mulheres (65%), heterossexuais (97%), católicos (97%), pescadores (42%), com idade média de 48 anos. Realizou-se entrevistas semiestruturadas, analisadas no software IRaMuTeQ, versão 0.7 alpha 2, pelo método de Classificação Hierárquica Descendente. Identificou-se representações dicotômicas acerca dos construtos estudados, que carregam indícios de preconceito sutil direcionado a idosos LGBTI’s, bem como, uma compreensão biologizante do envelhecimento. Discutiu-se os resultados, corroborando e/ou os contrapondo com estudos anteriores. É esperado que este estudo contribua para o desenvolvimento de investigações futuras, e para a criação e implementação de políticas públicas direcionadas a essa população.

Abstract: Oriented Strand Board is a structural wood composite with applications that require good physical and mechanical performance. The addition of ZnO nanoparticles is an alternative that has been studied in order to improve the properties of Oriented Strand Board panels. However, there is no information about its effect Oriented Strand Board. The aim of this work was to evaluate the influence of the addition of zinc oxide nanoparticles in two different percentages (0,25 % and 0,50 %) on the physical-mechanical properties of Oriented Strand Board panels produced with phenol-formaldehyde resin and on the heat transfer during hot-pressing. Oriented Strand Board panels were produced and tested according to European Standards. The addition of ZnO nanoparticles improved the dimensional stability of the panel, reducing its thickness swelling, and also increased the screw withdraw strength. The heat transfer during hot-pressing increased the temperature more quickly on boards with nanoparticles addition; on the other hand the final temperature of the control treatment was higher.

ABSTRACT Protein quality is related to amino acid composition and digestibility. Accurate evaluation of apparent digestibility coefficients (ADCs) of nutrients in commonly used feedstuffs is paramount for formulating efficient aquafeed. ADCs of soybean meal (SBM) and poultry by-product meal (PBM) were evaluated using reference diets formulated with two types of ingredients (semi-purified [SP] and practical [P]) for juvenile Nile tilapia ( Oreochromis niloticus, Linnaeus) of the GIFT strain. Groups of 20 juveniles (65.05 ± 12.37 g) were fed twice a day to apparent satiety with one of the four experimental diets (SBM-SP, SBM-P, PBM-SP, and PBM-P) in quadruplicate for 30 days. After the last feeding, feces were collected by siphoning hourly and the ADCs of dry matter, protein, and amino acids (AAs) were calculated. Nile tilapia exhibited a high capacity to digest SBM and PBM, with most ADCs exceeding 90 %. The type of reference diet affected the ADCs of protein and AAs on the test ingredients, with the SP reference diet providing the highest ADC, mainly in SBM. Digestibility data generated with a P-type reference diet demonstrated more practical relevance than those generated with an SP-type reference diet. They can be applied in digestibility studies for Nile tilapia.

Resumen Antecedentes: el número de personas mayores en el mundo está creciendo significativamente, por lo que urge la necesidad de asegurar que esta población pueda vivir una vida plena, especialmente, teniendo en cuenta el actual escenario pandémico de COVID-19. Objetivo: analizar las representaciones sociales de la vejez LGBT para los trabajadores del sexo masculino y femenino frente a la pandemia. Método: se trata de una investigación cualitativa, descriptiva y exploratoria. Participaron 10 hombres y 10 mujeres, de entre 18 y 48 años, todos profesionales del sexo. Se utilizaron cuestionarios sociodemográficos y entrevistas semiestructuradas, realizadas mediante Google Forms y posteriormente analizadas mediante el método de Clasificación Jerárquica Descendente, en el software IRaMuTeQ. Resultados: los análisis presentaron tres clases: (1) la comprensión social del tema de la vejez LGBT, (2) los estigmas sobre los géneros y las orientaciones sexuales y (3) los cambios físicos y los obstáculos que vienen con ellos. Conclusiones: las representaciones están asociadas a las dificultades que tienen las personas mayores para envejecer en sociedad, así como para debatir sobre la vejez LGBT. También existen obstáculos aún mayores para el envejecimiento de las mujeres profesionales del sexo. Así, el estudio anhela contribuir a la ampliación del conocimiento de los aspectos psicosociales de la vejez LGBT, en especial de los profesionales del sexo.

Abstract Background: The number of elderly people in the world is growing significantly, so there is an urgent need to ensure that this population can live a full life, especially taking into account the current COVID-19 pandemic scenario. Objective: To analyze the social representations of LGBT old age for male and female sex workers through the pandemic. Method: A qualitative, descriptive, and exploratory research conducted with 10 male and 10 female sex workers aged between 18 and 48. Sociodemographic questionnaires and semi-structured interviews were administered using Google Forms and then analyzed by descending hierarchical classification through the IRaMuTeQ software. Results: The analysis showed three classes: (1) «Social understanding of the topic LGBT old age,» (2) «Stigma of genders and sexual orientations,» and (3) «Physical transformations and their accompanying obstacles». Conclusions: The representations are associated with the difficulties that elderly people have with aging in society, as well as debating LGBT old age. There are also greater obstacles regarding aging of female sex workers. In this way, the study seeks to contribute to broaden the knowledge of the psychosocial aspects of LGBT aging and old age, especially those of sex workers.

Abstract The Brazilian Code ABNT NBR 7190 (1997) is currently under review process and one of the proposals is the change and add of some test procedures. Several specimen geometric configurations and test conditions have been evaluated by the study committee CE-02:126.10. The aim of this paper was the analysis of the tensile strength perpendicular to the grain through the current Brazilian Code and from an adaptation of ISO 13910 (2005). Pine and Indian Cedar were used and simplified relationships were proposed. Numerical simulations were performed to identify the stress distributions in the specimens. The results obtained by ISO 13910 (2005) did not differ statistically for the two species. Brazilian standard method cedar had a higher strength value than pine. The simplified ratios obtained ranged between 0,017 and 0,123. The numerical simulations of ISO 13910 (2005) specimens showed predominant failure by tension in the lower fiber due to stress concentration.

Resumo Painéis OSSB (Oriented Structural Straw Board) são compósitos produzidos com palha proveniente de resíduos agrícolas e utilizando de adesivo sem formaldeído, cuja principal aplicação se volta para a construção civil como material de fechamento e forros. Este trabalho teve como objetivo avaliar o efeito do teor de poliuretano no adesivo sobre propriedades físicas e mecânicas do OSSB produzido com SbS (palha de soja) localmente disponível. Quatro composições de poliuretano à base de óleo de mamona (6%, 9%, 12% e 15%, em relação à massa de palha) foram testadas. Painéis OSSB foram avaliados de acordo com os padrões europeus para compósitos à base de madeira; também, uma caracterização física e química da SbS foi realizada. A SbS possui formato irregular, o que reduziu o desempenho mecânico do OSSB devido à baixa densificação do compósito e ao desenvolvimento de trincas transversais na SbS após o processo de fabricação do compósito. O desempenho em testes de flexão estática e inchamento de espessura do OSSB não foi melhorado pelo aumento do teor de adesivo de poliuretano. A SbS apresentou baixo teor de cinzas (menos que 4.3%) e pH neutro, isto indica que estas propriedades químicas provavelmente não afetaram na cura do adesivo.

Abstract Oriented Structural Straw Board (OSSB) panels are composites made from straw originally from agricultural residues and non-formaldehyde based adhesive, whose main application is for construction as a closing and ceiling material. The objective of this study was to evaluate the effect of the polyurethane adhesive content on physical and mechanical properties of OSSB produced with locally available SbS (soybean straw). Four castor oil based polyurethane ratios (6%, 9%, 12% and 15%, by mass of the SbS) were tested. OSSB was evaluated according to European standards for wood-based composites; also, a physical and chemical characterization of the SbS was performed. SbS has an irregular shape, which reduced the OSSB mechanical performance due to the low densification of the composite and the development of transversal cracks on the SbS after the OSSB manufacturing process. The static bending and thickness swelling performance of SbS OSSB was not improved by the increase of polyurethane adhesive content. SbS low ash content and neutral pH extractives indicates that probably its chemical properties should not affect the setting of the adhesive.

RESUMO Objetivo Verificar indicadores de desenvolvimento cognitivo, habilidades de linguagem receptiva e padrões comportamentais adaptativos em pré-escolares com Síndrome de Williams (SW). Método A amostra foi composta por 8 crianças de ambos os sexos, com idade entre 48 e 72 meses com SW. Os instrumentos de coleta de dados utilizados foram Denver Developmental Screening Test II; Peabody Picture Vocabulary Test; Escala de comportamento adaptativo Vineland; Child Behavior Checklist (CBCL) para as idades 1½-5 e 6 a 18; Inventário de Problemas de Comportamento (BPI-01) e Escala de Maturidade Mental Columbia. Resultados Os principais prejuízos de desenvolvimento foram associados a habilidades motoras finas e habilidades de cuidados pessoais. Os déficits em linguagem receptiva e habilidades de comunicação foram relatados de acordo com o PPVT e Denver II, respectivamente. Os cuidadores relataram problemas comportamentais e emocionais associados às escalas de ansiedade e depressão e de problemas de atenção do CBCL. Conclusão Os pré-escolares demonstraram déficits no funcionamento adaptativo e dificuldades comportamentais, motoras e cognitivas, como desatenção e hiperatividade, estereotipias e comportamento agressivo.

ABSTRACT Purpose To verify indicators of cognitive development, receptive language skills and adaptive behavioral patterns in toddlers with Williams syndrome (WS). Methods The sample comprised 8 children of both sex, aged between 48 and 72 months with WS. Instruments of data collection were Denver Developmental Screening Test II; Peabody Picture Vocabulary Test; Vineland Adaptive Behavior Scale; Child Behavior Checklist for Ages 1½-5and 6 to 18; Columbia Mental Maturity Scale (CMMS), and Behavior Problems Inventory-01. Results The major developmental impairments were associated with fine motor skills and personal care abilities. Deficits in receptive language and communication skills were reported according to the PPVT and Denver II, respectively. The caregivers reported behavioral and emotional problems associated to anxiety and depression, and attention problems scales of CBCL. Conclusion The toddlers demonstrated deficits in adaptive functioning and behavioral, motor and cognitive difficulties such as inattention and hyperactivity, stereotypies and aggressive behavior.

The addition of a capped mini-exon [spliced leader (SL)] through trans-splicing is essential for the maturation of RNA polymerase (pol) II-transcribed polycistronic pre-mRNAs in all members of the Trypanosomatidae family. This process is an inter-molecular splicing reaction that follows the same basic rules of cis-splicing reactions. In this study, we demonstrated that mini-exons were added to precursor ribosomal RNA (pre-rRNA) are transcribed by RNA pol I, including the 5' external transcribed spacer (ETS) region. Additionally, we detected the SL-5'ETS molecule using three distinct methods and located the acceptor site between two known 5'ETS rRNA processing sites (A' and A1) in four different trypanosomatids. Moreover, we detected a polyadenylated 5'ETS upstream of the trans-splicing acceptor site, which also occurs in pre-mRNA trans-splicing. After treatment with an indirect trans-splicing inhibitor (sinefungin), we observed SL-5'ETS decay. However, treatment with 5-fluorouracil (a precursor of RNA synthesis that inhibits the degradation of pre-rRNA) led to the accumulation of SL-5'ETS, suggesting that the molecule may play a role in rRNA degradation. The detection of trans-splicing in these molecules may indicate broad RNA-joining properties, regardless of the polymerase used for transcription.

The parasitic protozoan Leishmania (Leishmania) amazonensis alternates between mammalian and insect hosts. In the insect host, the parasites proliferate as procyclic promastigotes andthen differentiate into metacyclic infective forms. The meta 1 gene is preferentially expressed during metacyclogenesis. Meta 1 expression profile determination along parasite growth curves revealed that the meta 1 mRNA level peaked at the early stationary phase then decreased to an intermediate level. No correlation was observed between meta 1 expression and infectivity. Conversely, infectivity correlated with the increase of apoptotic cells in the late stationary phase.

O estudo de estabilidade de azitromicina em preparações oftálmicas foi realizado após exposição a diferentes tipos de luz, temperatura e pH, utilizando o método de difusão em ágar (cilindros 3 x 3) para as quantificações. A faixa de concentração foi de 50 a 200 µg/mL. O estudo demonstrou que o fármaco sofreu degradação quando submetido às luzes ultravioleta, germicida e solar, e a soluções ácida, alcalina e de peróxido de hidrogênio. Os resultados foram analisados através da análise da variância (ANOVA).

A stability study of azithromycin in ophthalmic preparations was developed by submission to different types of light, temperature and pH, using the biodiffusion assay (cylinder 3 x 3) for the quantifications. Bacillus subtilis, ATCC 9372, was used as test organism. The used concentration range was of 50 to 200 µg/mL. The study demonstrated that the drug suffered degradation when submitted to the ultraviolet light, germicide light, solar luminosity, acid solution, basic solution and hydrogen peroxide solution. The results were analyzed by the analysis of variance (ANOVA).

O presente estudo teve como objetivo realizar a adaptação transcultural de um instrumento de qualidade de vida, específico para pacientes neurocirúrgicos, denominado Innsbruck Health Dimensions Questionnaire for Neurosurgical Patients (IHD-NS). Participaram deste estudo 30 pacientes, de ambos os sexos, que foram submetidos a cirurgia de tumor cerebral há mais de doze meses, com idade variando entre 26 e 66 anos. Após o processo de tradução/retro-tradução e elaboração da versão brasileira do instrumento, os pacientes foram avaliados e reavaliados após um intervalo de um mês. As análises estatísticas evidenciaram preservação da consistência interna, índices de concordância elevados e correlação intraclasse altamente significativa permitindo considerar boa a qualidade e a confiabilidade da versão em português, denominada Questionário de Dimensões de Saúde para Pacientes Neurocirúrgicos de Innsbruck - DSI (NC).

The goal of this study was to accomplish the cross-cultural adaptation of a quality of life instrument, specific for neurosurgical patients, called Innsbruck Health Dimensions Questionnaire for Neurosurgical Patients (IHD-NS). Thirty patients participated in this study, male and female, all having been submitted to brain tumor surgery more than twelve months before, and whose ages ranged from 26 to 66. After the process of translation/back translation and the elaboration of the Brazilian version of the instrument, the patients were assessed and reassessed within a one-month period. Statistical analyses evinced the preservation of the internal consistency, high agreement levels and highly significant intra-class correlation, allowing for the belief in the quality and reliability of the Portuguese version, named Questionário de Dimensões de Saúde para Pacientes Neurocirúrgicos de Innsbruck - DSI (NC).

OBJETIVO: A Síndrome de Hipoplasia de Câmaras Esquerdas representa um grande desafio para cirurgiões do mundo inteiro. Atualmente, tem sido proposto procedimento paliativo alternativo, por meio da bandagem bilateral das artérias pulmonares associada à colocação de stent no canal arterial e atrioseptostomia. No entanto, as bandagens utilizadas são fixas, podendo tornar-se inadequadas após o fechamento do esterno ou com o rápido crescimento somático do paciente. Descrevemos a primeira aplicação clínica do novo dispositivo miniaturizado de bandagem ajustável das artérias pulmonares em neonato portador da síndrome de hipoplasia de câmaras esquerdas, o qual permitiu ajustes percutâneos precisos do fluxo sangüíneo pulmonar. MÉTODO: Através de esternotomia mediana, neonato de 5 dias de vida foi submetido à bandagem pulmonar bilateral, usando este novo dispositivo, combinada com interposição de tubo de PTFE entre o tronco pulmonar e o tronco braquiocefálico. RESULTADOS: O paciente apresentou boa evolução pós-operatória. Três ajustes percutâneos das bandagens foram necessários para manter a saturação arterial de oxigênio entre 75-85%. No 48º dia de vida, o paciente foi submetido a atrioseptostomia com colocação de stent (6 mm) para tratamento de comunicação interatrial restritiva. No 106º dia de vida, realizou-se operação de Norwood associada à anastomose cavopulmonar bilateral. As bandagens foram removidas, sem distorção das artérias pulmonares. CONCLUSÕES: O uso clínico deste sistema inovador de bandagem ajustável das artérias pulmonares mostrou-se factível, seguro e eficaz. Permitiu o ajuste fino do fluxo pulmonar de acordo com as necessidades clínicas, proporcionando um equilíbrio preciso entre as circulações pulmonar e sistêmica.

OBJECTIVE: Hypoplastic left heart syndrome remains a challenge for worldwide surgeons. Initial palliation employing bilateral pulmonary artery banding along with ductal stent implantation and atrial septostomy has been proposed as an alternative approach. However, the surgically placed bands are fixed and may become inadequate after sternum closure or with somatic growth of the patient. We describe the first case in which a neonate with hypoplastic left heart syndrome was initially managed using a mini banding system that allows for fine percutaneous adjustments of pulmonary blood flow. METHOD: Through a mid sternotomy, a 5 day-old neonate underwent bilateral pulmonary artery banding using this new system combined with placement of a main pulmonary artery to innominate artery shunt. RESULTS: The patient had an uneventful postoperative course. Three percutaneous adjustments of the banding system were necessary to keep the arterial oxygen saturation in the 75%-85% range. On the 48th day of life, she was submitted to stent placement (6 mm) within the atrial septum to treat a restrictive atrial septal defect. The Norwood operation and the bidirectional Glenn shunt were carried out on the 106th day of life. The bands were removed with no distortion of the pulmonary arteries. CONCLUSIONS: The clinical use of this innovative pulmonary artery banding system was feasible, safe and effective. It allowed for customization of the pulmonary blood flow according to the underlying clinical needs, resulting in a more precise balance between the pulmonary and systemic circulations.